Biomek Seed Dispense

Oh,

And the Biomek can also dispense seed for two ecotypes into a single well of a 24-well plate. Pics will be posted.

Span-8 Tip eject errors

The span-8 had a tip ejection error. The tip detectors said tips were still loaded after a tip eject, but they were not. Phil the Beckman guy was called to fix the problem.

The problem was specifically with probe 1, and it occured intermittently. Phil opened the system up and replaced the tip detection switch, but that wasn't necessarily the problem. He also opened up some board and reseated the tip detect wires. This rectified the problems and the span-8 has run without tip detect errors since then.

First Post

Note: This blog will be in a rudimentary form until it proves utility.
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Issues of concern for Biomek:

Precision and accuracy have been in question for the Biomek by new Users. It pays to be skeptical, but it's more work. I recently received an email from pjarcher@beckman.com conerning this issue. Both the 96-channel and the span-8 pod should be tested before the machine is used for aliquoting and dilutions.

The technician detailed the following info concerning the Span-8:


Span-8 Pipettor %CV Precision Accuracy
- 60mm probe 1uL dispense (wet well) 1mL syringe 8% 10%
- 60mm probe 5uL dispense 1mL syringe 4% 6%
- P20 tip 1uL dispense 1mL syringe 6% 7%
- P20 tip 1uL dispense 250uL syringe 6% 7%
- P200 tip 5uL dispense 1mL syringe 4% 6%

96-Multichannel pod




This issue will be verified independently and continuously investigated.


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Links to check out:

http://www.beckmancoulter.com/resourcecenter/labresources/automatedsolutions/bmk_pipette.html
http://forums.beckmancoulter.com/index.php?showforum=4

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Pics from my first large scale aliquot:


Input:



Output: